Detergents belonging to different classes, such as ionic and non-ionic detergents, can exhibit strongly differing properties. To optimize detergent properties for a given application, ionic and non-ionic detergents are often non-covalently mixed. To understand how combining ionic and non-ionic detergent headgroups through a covalent bond influences detergent properties, I herein investigated ionic/non-ionic hybrid detergents regarding their critical micelle concentrations (cmc), encapsulation efficiency, hard water tolerance and antibacterial properties. To determine cmc values, I compared two dynamic light scattering approaches, i.e. concentration-dependant analysis of light scattering intensity and diffusion coefficients. My results reveal that the concentration-dependant diffusion coefficient analysis deliveres reproducible cmc values, while light scattering intensity analysis is unsuitable for this detergent class. Properties of ionic/non-ionic hybrid detergents depend on several structural features, including the charge of the ionic headgroup, size of the non-ionic headgroup and distance between ionic headgroup and non-polar tail. Overall, shielding the charge of an ionic headgroup with the help of a neighbouring non-ionic headgroup decreases cmc values, increases encapsulation efficiency and hard water tolerance, and reduces antibacterial properties. My results uncover that ionic/non-ionic hybrid detergents exhibit scalable properties and great potential to be used as nanocarriers in future applications.